anti vascular cell adhesion molecule Search Results


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Epizyme Inc antibodies against vascular cell adhesion molecule 1
Rg1 pretreatment modulates the immunophenotype of adipose-derived mesenchymal stromal cells (ADSCs). A Representative microscopy image showing the morphological features of ADSCs after ginsenoside Rg1 treatment (200×). B CCK-8 assay assessing ADSC proliferation following Rg1 pretreatment (0–100 µM). ** p < 0.01, compared with 0 µM group. C Quantitative reverse transcription RT-qPCR (Reverse Transcription quantitative PCR) analysis of stemness-related genes (Nanog, SOX2, OCT4) following Rg1 exposure. D , E Flow cytometry analysis of cell cycle distribution in ADSCs with or without Rg1 treatment. F - I Immunofluorescence staining for vascular cell <t>adhesion</t> <t>molecule-1</t> (VCAM1, green, 200×) and intercellular adhesion molecule-1 (ICAM1, red, 200×) to assess immunorecruitment capacity. J , K Flow cytometric profiling of immune-related surface markers, including HLA-DQ, HLA-DR, HLA-G, CD59, and CD73, in response to Rg1 pretreatment. Data are presented as mean ± standard deviation (SD), n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, ns, not significant, compared with untreated ADSCs
Antibodies Against Vascular Cell Adhesion Molecule 1, supplied by Epizyme Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibodies against vascular cell adhesion molecule 1 - by Bioz Stars, 2026-10
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Rg1 pretreatment modulates the immunophenotype of adipose-derived mesenchymal stromal cells (ADSCs). A Representative microscopy image showing the morphological features of ADSCs after ginsenoside Rg1 treatment (200×). B CCK-8 assay assessing ADSC proliferation following Rg1 pretreatment (0–100 µM). ** p < 0.01, compared with 0 µM group. C Quantitative reverse transcription RT-qPCR (Reverse Transcription quantitative PCR) analysis of stemness-related genes (Nanog, SOX2, OCT4) following Rg1 exposure. D , E Flow cytometry analysis of cell cycle distribution in ADSCs with or without Rg1 treatment. F - I Immunofluorescence staining for vascular cell adhesion molecule-1 (VCAM1, green, 200×) and intercellular adhesion molecule-1 (ICAM1, red, 200×) to assess immunorecruitment capacity. J , K Flow cytometric profiling of immune-related surface markers, including HLA-DQ, HLA-DR, HLA-G, CD59, and CD73, in response to Rg1 pretreatment. Data are presented as mean ± standard deviation (SD), n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, ns, not significant, compared with untreated ADSCs

Journal: Stem Cell Research & Therapy

Article Title: Rg1-preconditioned adipose-derived mesenchymal stromal cells alleviate colitis via exosome-mediated Inhibition of macrophage glycolysis through RAS signaling

doi: 10.1186/s13287-025-04822-4

Figure Lengend Snippet: Rg1 pretreatment modulates the immunophenotype of adipose-derived mesenchymal stromal cells (ADSCs). A Representative microscopy image showing the morphological features of ADSCs after ginsenoside Rg1 treatment (200×). B CCK-8 assay assessing ADSC proliferation following Rg1 pretreatment (0–100 µM). ** p < 0.01, compared with 0 µM group. C Quantitative reverse transcription RT-qPCR (Reverse Transcription quantitative PCR) analysis of stemness-related genes (Nanog, SOX2, OCT4) following Rg1 exposure. D , E Flow cytometry analysis of cell cycle distribution in ADSCs with or without Rg1 treatment. F - I Immunofluorescence staining for vascular cell adhesion molecule-1 (VCAM1, green, 200×) and intercellular adhesion molecule-1 (ICAM1, red, 200×) to assess immunorecruitment capacity. J , K Flow cytometric profiling of immune-related surface markers, including HLA-DQ, HLA-DR, HLA-G, CD59, and CD73, in response to Rg1 pretreatment. Data are presented as mean ± standard deviation (SD), n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, ns, not significant, compared with untreated ADSCs

Article Snippet: Samples were then incubated overnight at 4 °C with primary antibodies against vascular cell adhesion molecule-1 (VCAM-1, 1:200, 28L12K31), intercellular adhesion molecule-1 (ICAM-1, 1:200, 33M53E27) (both from Epizyme, China), and iNOS (22226-1-AP, Proteintech, China, 1:300), Arginase-1 (16001-1-AP, Proteintech, China, 1:300), LGR5 (30007-1-AP, Proteintech, China, 1:300), F4/80 (28463-1-AP, Proteintech, China, 1:300), and GLUT1 (21829-1-AP, Proteintech, China, 1:300).

Techniques: Derivative Assay, Microscopy, CCK-8 Assay, Reverse Transcription, Quantitative RT-PCR, Real-time Polymerase Chain Reaction, Flow Cytometry, Immunofluorescence, Staining, Standard Deviation