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Journal: Stem Cell Research & Therapy
Article Title: Rg1-preconditioned adipose-derived mesenchymal stromal cells alleviate colitis via exosome-mediated Inhibition of macrophage glycolysis through RAS signaling
doi: 10.1186/s13287-025-04822-4
Figure Lengend Snippet: Rg1 pretreatment modulates the immunophenotype of adipose-derived mesenchymal stromal cells (ADSCs). A Representative microscopy image showing the morphological features of ADSCs after ginsenoside Rg1 treatment (200×). B CCK-8 assay assessing ADSC proliferation following Rg1 pretreatment (0–100 µM). ** p < 0.01, compared with 0 µM group. C Quantitative reverse transcription RT-qPCR (Reverse Transcription quantitative PCR) analysis of stemness-related genes (Nanog, SOX2, OCT4) following Rg1 exposure. D , E Flow cytometry analysis of cell cycle distribution in ADSCs with or without Rg1 treatment. F - I Immunofluorescence staining for vascular cell adhesion molecule-1 (VCAM1, green, 200×) and intercellular adhesion molecule-1 (ICAM1, red, 200×) to assess immunorecruitment capacity. J , K Flow cytometric profiling of immune-related surface markers, including HLA-DQ, HLA-DR, HLA-G, CD59, and CD73, in response to Rg1 pretreatment. Data are presented as mean ± standard deviation (SD), n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, ns, not significant, compared with untreated ADSCs
Article Snippet: Samples were then incubated overnight at 4 °C with primary
Techniques: Derivative Assay, Microscopy, CCK-8 Assay, Reverse Transcription, Quantitative RT-PCR, Real-time Polymerase Chain Reaction, Flow Cytometry, Immunofluorescence, Staining, Standard Deviation